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KMID : 1161520150190020127
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Animal Cells and Systems
2015 Volume.19 No. 2 p.127 ~ p.132
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Biochemical functions of integrase-binding domain of lens epithelium-derived growth factor
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Kim Jin-Sun
Hamid Faysal Bin
Shin Cha-Gyun
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Abstract
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Integrase-binding domain (IBD) is a critical region on the lens epithelium-derived growth factor (LEDGF/p75) protein that specifically interacts with human immunodeficiency virus type 1 integrase (HIV-1 IN). In this study, we expressed and purified the IBD, and utilized in endonucleolytic assay and strand transfer assay to investigate whether IBD improve enzymatic activities of HIV-1 IN. Our results showed that IBD along with HIV-1 IN increased the strand transfer activity but not the endonucleolytic activity. Furthermore, our results showed that IBD induced efficient integration of the donor DNA into the target DNA to create half-site integration and full-site integration using plasmid as target DNA. Therefore, it is suggested that LEDGF-IBD itself might be able to increase viral infectivity by enhancing the strand transfer activities of HIV-1 IN and accelerating integration of viral cDNA into cellular chromosomal DNA.
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KEYWORD
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lens epithelium-derived growth factor, human immunodeficiency virus type 1, integrase, integrase-binding domain
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